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KMID : 0360220110520121514
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Journal of the Korean Ophthalmological Society
2011 Volume.52 No. 12 p.1514 ~ p.1518
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Effect of Erythropoietin on the Production of Nitric Oxide in Trabecular Meshwork Cells
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Lee Seung-Hee
Kim Jae-Woo
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Abstract
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Purpose: To investigate the effects of erythropoietin (EPO) on the production of nitric oxide in cultured human trabecular meshwork cells (HTMC).
Methods: Primarily cultured HTMC were exposed to 0, 0.5, and 1.0 U/ml EPO using serum-deprived media for 2 days. Production of nitric oxide and cellular survival were assessed with Griess assay and MTT assay, respectively. Expression of EPO mRNA receptor and activity of eNOS were assessed with RT-PCR.
Results: EPO did not affect the survival of HTMC (p > 0.05). EPO increased the production of nitric oxide (p < 0.05) accompanied with increased eNOS activity.
Conclusions: EPO had no effect on the cellular survival under serum-deprived conditions. EPO increases the production of nitric oxide by increasing eNOS activity.
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KEYWORD
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Erythropoietin, Nitric oxide, Trabecular meshwork cells
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